fluoroSENS - fluorimeter

Versatile bench-top fluorimeter

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The fluoroSENS is a versatile bench-top fluorimeter with single photon counting sensitivity and an excellent water Raman signal-to-noise ratio. It combines measurements of both high performance steady state fluorescence spectra and time resolved phosphorescence decays. The fluoroSENS is a fully integrated, computer controlled single photon counting fluorimeter with comprehensive optional accessories.

The light source is a highly stabilised Xenon arc lamp with an optical stability better than 0.1% with an auto-start facility in instrument power ON.

The excitation and emission monochromators are designed for high optical throughput and each has a triple grating turret for flexible choice of gratings. This allows the instrument to be optimised for several spectral ranges depending upon customer requirements.  Each monochromator is also fitted with continuously variable bilateral knife edge slits allowing spectral pass band from 0.1nm to 10mm.

FluoroSENS is fitted with several key spectroscopy features as standard.  These include a reference photodiode detector for corrected excitation spectra, corrected emission channel, integrated order sorting filter wheel and filters as well as an optical attenuator to allow signal optimisation.

As standard the large sample chamber is designed with high light gathering optics to utilise as much of the sample signal as possible.  AS standard the fluoroSENS comes with a singel cuvette holder on a positioning stage to ensure sample and signal optimisation.  Optional samples holders include for example: temperature controlled options, four cell motorised turret, front face sample holder for solid or power samples, magnetic stirrer, optical fibre adapters and a motorised 96 well plate reader.

Each fluoroSENS uses a single photon sensitive photomultiplier detector. This detector can be exchanged for different spectral ranges to ensure complete optimisation to customer needs. Optional NIR detectors are also available.

The fluoroSENS uses a new class leading data acquisition system that provides all signal collection in the one board.  This includes a 100M counts per second photon counting module as well as low noise 16 bit analogue-to-digital detectors for sample temperature sensing and the reference detectors.

Uniquely, fluoroSENS offers a transmission detector as a sample chamber option to allow the measurement of sample transmission simultaneously with the fluorescence emission or excitation measurements.  This allows the user to directly monitor sample photo-bleaching or aggregation etc that might otherwise invalidate the fluorescence measurement.

The system software contains all of the control, data collection, and analysis functions to make high quality fluorescence measurements.  It has the option to save all experimental parameters and to define a quick load measurement function.  This function permits routine measurements to be set-up and stored for repeat use.  Full flexibility is offered to edit these functions.

Spectral functions provided as standard include:

  • Exc, Em, and Synchronous Spectra
  • Anisotropy
  • Ex-Em Mapping, Synchronous Mapping Spectra
  • 2D, 3D, and contour plotting
  • Spectral Arithmetic, Normalisation, Smoothing, Differentiation, Integration, Spectral Conversion, Anisotropy Analysis (incl. G-factor correction), etc
  • Comprehensive export facilities for the data and the graphical presentations

The fluoroSENS also has a flexible range of upgrade paths that include:

  • pulsed Xe lamp for phosphorescence spectra and lifetimes
  • Silicon photo-diode transmission detector
  • Low temperature sample options including nitrogen and helium cryostats
  • stop-flow accessory
  • optical fibres for remote sensing applications
  • 96 well plate reader accessory

Product Image

Specifications

Optical Configuration Right angle geometry, optical accessories for other geometries
Excitation Source 150W continuous Xenon Arc Lamp, Ozone Free
200 to 2600nm spectral range
XYZ  bulb, rear mirror and lamp focus adjustments – factory pre-aligned
Monochromators
XYZ  bulb, rear mirror and lamp focus adjustments – factory pre-aligned
Monochromators Czerny- Turner design
300mm focal length
Triple grating turret as standard
Motorised continuously variable knife edge slits form 10µm to 3mm, as standard
Stray Light performance 1:105
Excitation Monochromator – blaze @ 300nm, 1200g/mm
Emission Monochromator – blaze @ 500nm, 1200g/mm
Detector 185 – 670nm, blue sensitive singel photon counting photomultiplier.  Dark count rate <100cps at room temperature
185 – 870nm, red sensitive singel photon counting photomultiplier.  Dark count rate <2000cps at room temperature
185 – 1000nm, large area calirated Silicon Photodiode reference detector
Optional InGaAs, InAs, InSb emission detectors
Data Aquisition 100M counts per second photon counting system
4 x 16 bit, 1Ms/s digitisers for analogue detectors and sample temperature monitor
flexible user I/O port
Software Operating System – WINDOWS®
Exc, Em, Synchronous, Anisotropy, Ex-Em Mapping, Synchronous Mapping Spectra, 2D, 3D, and contour plotting
Spectral Arithmetic, Normalisation, Smoothing, Differentiation, Integration, Spectral Conversion, Anisotropy Analysis (incl. G-factor correction)
Sensitivity Water Raman signal-to-noise ration > 2500:1
Excitation wavelength – 350nm, spectral bandwidth – 5nm, Integration Time = 1s
tel: +44(0)141.952.9475   fax: +44(0)141.952.9400   mbl: +44(0)798.357.6530   sales@gildenphotonics.com   
Gilden Photonics Ltd., Unit 13 Telford Court, 9 South Avenue, Clydebank Business Park, Glasgow, G81 2NR, UK

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